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KMID : 1059519840280030185
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Journal of the Korean Chemical Society
1984 Volume.28 No. 3 p.185 ~ p.193
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Change in the Binding Cooperativity of Ethidium Calf Thymus DNA, Induced by Spermine Binding
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Ko Thong-Sung
Huh Joon
Lee Chan-Yong
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Abstract
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At the spermine concentration to cover the number of the binding site of spermine 0.016 per nucleotide, the Hill coefficient of the ethidium binding to the calf thymus DNA as 1.7, while the value was 0.38 in the absence of the spermine. On the basis of the data, together with other present data on the viscometric titration of the DNA with spermine and anomalous absorbance-temperature profile at 260nm and viscosity-temperature profile, it can be speculated that allosteric propagation of the conformational transition induced by the binding of the spermine may be involved in the monomolecular collapse of the DNA to a condensed structure.
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